DIAGNOSIS OF GONOCOCCAL URETHRITIS AND CHLAMYDIAL URETHRITIS BY POLYMERASE CHAIN REACTION
نویسندگان
چکیده
منابع مشابه
[Diagnosis of gonococcal urethritis and chlamydial urethritis by polymerase chain reaction].
A polymerase chain reaction (PCR) method was compared to standard methods (cultures for Neisseria gonorrhoeae and Chlamydia trachomatis and an enzyme-immunoassay for C. trachomatis) in diagnosis of gonococcal and chlamydial urethritis in 40 male patients with urethritis. Gonococcal urethritis was diagnosed by detection of a 206 bp DNA fragment amplified by PCR with N. gonorrhoeae-specific prime...
متن کاملPolymerase chain reaction is no better than Gram stain for diagnosis of gonococcal urethritis.
Sir, Chlamydia trachomatis and Neisseria gonorrhea are the leading reportable causes for sexually transmitted diseases (STDs) in the United States.[1,2] In UK, gonorrheal disease is considered the second most common sexually transmitted infection of bacterial origin.[3] In France, the average number of Neisseria gonorrhea isolated per laboratory per year decreased from 10.6 in 1986 to 0.6 in 19...
متن کاملDetection of Chlamydia trachomatis DNA in patients with non-gonococcal urethritis using the polymerase chain reaction.
A practical protocol using the polymerase chain reaction (PCR) was designed for detecting Chlamydia trachomatis in clinical samples. DNA was extracted from material collected on urethral swabs and used as substrate for the PCR. The target was a 600 basepair DNA segment of the multicopy plasmid that is common to all strains of the bacterium. Negative samples were checked for loss of DNA or prese...
متن کاملDiagnosis of Chlamydia trachomatis urethritis in men by polymerase chain reaction assay of first-catch urine.
To determine the accuracy of a recently developed polymerase chain reaction (PCR) urine assay to detect Chlamydia trachomatis urethral infection in men, we obtained urethral swabs and first-catch urine from 365 men attending a sexually transmitted diseases clinic. Thirty-three (9%) of the 365 men were infected with C. trachomatis as defined by urethral culture. Thirty-two of the 33 men with cul...
متن کاملManagement of non-gonococcal urethritis
Non-gonococcal urethritis (NGU), or inflammation of the urethra, is the most common treatable sexually transmitted syndrome in men, with approximately 20-50 % of cases being due to infection with Chlamydia trachomatis and 10-30 % Mycoplasma genitalium. Other causes are Ureaplasma urealyticum, Trichomonas vaginalis, anaerobes, Herpes simplex virus (HSV) and adenovirus. Up to half of the cases ar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Japanese Journal of Urology
سال: 1992
ISSN: 0021-5287,1884-7110
DOI: 10.5980/jpnjurol1989.83.2070